Journal: Retrovirology

Article Title: Increased BST2 expression during simian immunodeficiency virus infection is not a determinant of disease progression in rhesus monkeys

doi: 10.1186/s12977-015-0219-8

Figure Lengend Snippet: BST2 mRNA induction by type I interferon. a Fold induction of relative BST2 mRNA in PBMC from three uninfected rhesus macaques after stimulation with human Interferon Alpha A (Alpha 2a) for 16 h. Data are expressed as fold increase over baseline after normalization to pre-treatment values. Error bars represent standard deviation, b relative mRNA copies of BST2 in PBMC (shown in copy numbers per 100 copies of GAPDH) are illustrated in relation to plasma IFN-alpha levels from blood samples of 18 uninfected rhesus macaques 24 h after inoculation of replication incompetent adenovirus or fowl pox vectors. The black dashed line indicates the detection limit of the ELISA and c whole blood MX1 mRNA levels correlate with BST2 mRNA determined in 38 SIVmac251 infected rhesus macaques at 24 wpi. Relative mRNA levels are depicted as log-transformed copy numbers per 100 copies of GAPDH. Each data point represents one animal. Regression line is shown; r , Spearman’s correlation coefficient; p , p value

Article Snippet: PBMC from three healthy animals were stimulated with 10, 50 and 100 ng Human Interferon Alpha A (Alpha 2a) (PBL Biomedical Laboratories) for 16 h. Fold induction of BST2 mRNA expression was calculated using GAPDH as internal control.

Techniques: Standard Deviation, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Infection, Transformation Assay

Journal: Cell Reports Methods

Article Title: Generation, expansion, gene delivery, and single-cell profiling in rhesus macaque plasma B cells

doi: 10.1016/j.crmeth.2024.100878

Figure Lengend Snippet:

Article Snippet: IFN alpha 2B , Proteintech , HZ-1072.

Techniques: Enzyme-linked Immunospot, Isolation, Enzyme-linked Immunosorbent Assay, Recombinant, Software

Journal: Virology

Article Title: Pulmonary C-fiber degeneration downregulates IFN-γ receptor 1 via IFN-α induction to attenuate RSV-induced airway hyperresponsiveness.

doi: 10.1016/j.virol.2017.06.034

Figure Lengend Snippet: Fig. 4. PCF degeneration downregulated RSV-induced IFNGR1 expression. (A) Immunohistochemistry staining. The left four pictures have a magnification of ×100 (bar = 100 µm), and the right four pictures are 400× magnification of the red boxes from the corresponding pictures on the left. (B) Western blot. The results are representative of three independent experiments, with a total of 5–7 mice/group, and all data are presented as the mean ± SEM. (*) P < 0.05, and (***) P < 0.001. Experiments were performed as described in Materials and Methods.

Article Snippet: Incubation with rabbit anti-IFNGR1 primary antibody was conducted overnight at 4 °C (1:100 dilution; Abcam, Cambridge, MA), followed by incubation with goat anti-rabbit secondary antibody (1:200 dilution; Boster, Wuhan, China) for 1 h at 37 °C.

Techniques: Expressing, Immunohistochemistry, Staining, Western Blot

Journal: Virology

Article Title: Pulmonary C-fiber degeneration downregulates IFN-γ receptor 1 via IFN-α induction to attenuate RSV-induced airway hyperresponsiveness.

doi: 10.1016/j.virol.2017.06.034

Figure Lengend Snippet: Fig. 5. IFNGR1 neutralization alleviated RSV-induced acute airway inflammation and AHR. Experiments were performed as described in Materials and Methods. (A) IFNGR1 neutralization reduced the number of total cells and (B) lymphocytes in BALF. (C) IFNGR1 neutralization alleviated RSV-induced pulmonary inflammatory cells infiltration. (D) IFNGR1 neutralization decreased RSV-induced airway resistance. (E) IFNGR1 neutralization did not affect the BALF IFN-γ levels. The results are representative of three independent experiments, with a total of 5–7 mice/group, and all data are presented as the mean ± SEM. (*) P < 0.05, (**) P < 0.01, and (***) P < 0.001. Fig (D): (**) P < 0.01, compared with RSV. Tc: Total cells, Mac: Macrophages, Lym: Lymphocytes, Neu: Neutrophils.

Article Snippet: Incubation with rabbit anti-IFNGR1 primary antibody was conducted overnight at 4 °C (1:100 dilution; Abcam, Cambridge, MA), followed by incubation with goat anti-rabbit secondary antibody (1:200 dilution; Boster, Wuhan, China) for 1 h at 37 °C.

Techniques: Neutralization

Journal: Virology

Article Title: Pulmonary C-fiber degeneration downregulates IFN-γ receptor 1 via IFN-α induction to attenuate RSV-induced airway hyperresponsiveness.

doi: 10.1016/j.virol.2017.06.034

Figure Lengend Snippet: Fig. 8. Exogenous recombinant mouse IFN-α (rIFN-α) downregulated RSV-induced IFNGR1 expression. Immunohistochemistry, qPCR and Western blot were done as described in Materials and Methods. (A) rIFN-α treatment induced STAT1 mRNA expression post-RSV infection. (B) rIFN-α treatment induced STAT1 and pSTAT1 protein expression post-RSV infection. (C) rIFN-α treatment reduced RSV-induced IFNGR1 expression detected by immunohistochemistry staining and (D) by Western blot. Magnification ×400, bar represents 100 µm. The results are representative of three independent experiments with a total of 6–8 mice/group, and all data are presented as the mean ± SEM. (*) P < 0.05, (**) P < 0.01, and (***) P < 0.001.

Article Snippet: Incubation with rabbit anti-IFNGR1 primary antibody was conducted overnight at 4 °C (1:100 dilution; Abcam, Cambridge, MA), followed by incubation with goat anti-rabbit secondary antibody (1:200 dilution; Boster, Wuhan, China) for 1 h at 37 °C.

Techniques: Recombinant, Expressing, Immunohistochemistry, Western Blot, Infection, Staining